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. 2014 Nov 10;6(6):1759091414558417. doi: 10.1177/1759091414558417

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Figure 8. — Detection of NSPCs apoptosis and intracellular ROS in vitro. (a–c) Images of the cells double-labeled for TUNEL (green) and DAPI (blue). Decreased cell apoptosis was observed in the edaravone-treated cells. Arrows indicated BrdU/TUNEL-double-positive cells. Bar = 50 µm. (d) Quantification of cell apoptosis. (e–g) Endogenous ROS was measured by flow cytometry. (h) Quantification of DCF fluorescence. The experiment was repeated three times. **p < .01 compared with the untreated group. ^##p < .01 compared with normoxia. TUNEL = terminal dUTP nick-end labeling; ROS = reactive oxygen species; DCF = 2,7-dichlorofluorescien.