Figure 4.

LPA1 receptor selective antagonist Ki16425 inhibits LPA effects on differentiation of hNP cells.

hNP cells were differentiated for 14 days, in the presence of 1 µM LPA or 10 µM Ki16425 for the final 10 days. Cell survival and protein expression were assessed as described. Cell viability (a) and DAPI-stained nucleus count (b) are reported as % of vehicle-treated, LIF control wells. βIII-tubulin (Tuj) expression (c) and Map2 expression (d) are reported as percent of differentiated cells expressing each marker above a set threshold.