Table 1.
Basic characteristics of the included studies
| Study | Country | Patients | Methods | Antibody source | CXCR4 distribution | Definition of CXCR4 positive |
|---|---|---|---|---|---|---|
| Zhou et al61 | PRC | 105 | IHC | Beijing Boao Sen | Cytoplasm | >5% |
| Otsuka et al51 | Canada | 170 | IHC | Biotrend | Cytoplasm | >10% |
| Wang et al60 | PRC | 208 | IHC | R&D | Cytoplasm | >5% |
| Chen et al62 | PRC | 64 | IHC | Abcam Ltd. | Membrane and/or cytoplasm | >5% |
| Minamiya et al63 | Japan | 79 | IHC/Western blot | Leinco Technologies | Nucleus or cytoplasm | >5% |
| Wagner et al44 | United States | 154 | IHC | R&D | Nucleus and cytomembrane | >+ |
| Na et al64 | Germany | 44 | IHC | R&D | Cytoplasm and nucleus | >1% |
| Su et al65 | PRC | 34 | IHC | R&D | Membrane and/or cytoplasm | Level of intensity |
| Suzuki et al59 | Japan | 236 | IHC | R&D | Cytoplasm and/or nucleus | >10% |
| Spano et al66 | France | 61 | IHC | Abcam Ltd. | Nucleus and/or cytoplasm | >10% |
| Hu et al67 | PRC | 66 | IHC/RT-PCR | Abcam Ltd. | Cytoplasm and/or nucleus | >5% |
| Zhang et al68 | PRC | 110 | IHC | R&D | Membrane and cytoplasm | >10% |
| Xiao et al69 | PRC | 115 | IHC | Boster | Membrane and cytoplasm | >10% |
Notes: Abcam Ltd.: Abcam Limited Biotechnology Europe, UK. Beijing Boao Sen: Beijing Boao Sen Biotechnology Co., Ltd. PRC. Biotrend: Biotrend Chemicals, LLC, Destin FL, USA. Boster: Boster, Pleasanton, CA, USA. Leinco Technologies: Leinco Technologies, Inc., St. Louis, MO, USA. R&D: R&D Systems, Inc., Minneapolis, MN, USA.
Abbreviations: IHC, immunohistochemistry; RT-PCR, reverse transcription polymerase chain reaction; PRC, People’s Republic of China; CXCR4, chemokine receptor type 4; +, positive.