Fig. 4.

Serum cytokine levels in the course of the case. Blood was sampled 1 week and 1 day before the first session of GMA, 2 days after the second session of GMA and 19 days after the last session of GMA. Concentrations of serum cytokines, IL-6, TNF-α, IL-1β and IL-8 were measured using Bio-Plex Pro human cytokine multiplex assay kits (Bio-Rad, Hercules, Calif., USA) and a Bio-Plex 200 System with high-throughput fluidics (Bio-Rad). The severity score of GPP for each day is indicated. The serum levels of IL-1 and IL-6 decreased with the improvement of GPP.