Table 1. CT values obtained from qPCR analysis of samples derived from TRIzol and the optimized RNA isolation approach.
Average CT value | |||
---|---|---|---|
Gene | P:C:IAA (optimized approach) | TRIzol | P-valuea |
hspX | 21.38 ± 0.20 | 23.60 ± 0.91 | 0.014 |
ethA | 27.34 ± 0.11 | 27.74 ± 1.13 | 0.608 |
fdxA | 20.62 ± 0.31 | 20.45 ± 0.56 | 0.663 |
relA | 23.82 ± 0.33 | 25.08 ± 0.89 | 0.081 |
sigA | 24.92 ± 0.15 | 24.70 ± 0.52 | 0.093 |
dosR | 23.73 ± 0.36 | 25.13 ± 0.82 | 0.053 |
dosS | 24.92 ± 0.32 | 26.53 ± 0.76 | 0.028 |
aAn unpaired Student's t-test was performed for the samples derived from the two approaches. Data represent mean ± SD for three biological replicates.