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. 2015 Jan 29;43(5):2864–2873. doi: 10.1093/nar/gkv009

Figure 1.

Figure 1.

Activity of BsMiniIII. (A) Cleavage of the Φ6 dsRNA substrate. Reactions aliquots were taken at 15, 30 and 45 min at 37°C. Each 10 μl aliquot contained 3.1 μg of wt BsMiniIII (8.3 μM) and 500 ng of Φ6 dsRNA. Samples were resolved on an agarose gel. The Ø indicates an untreated substrate. (B) Cleavage of the pKS-ACCU dsRNA substrate by BsMiniIII. Reactions were carried out for 1 h at 37°C with 125 nM BsMiniIII and 50 nM substrate. Samples were resolved on a native polyacrylamide gel. M: dsDNA molecular weight marker. The Ø indicates an untreated substrate. The nucleic acids were visualized after staining with ethidium bromide.