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. 2015 Feb 18;43(5):2543–2559. doi: 10.1093/nar/gkv083

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Phosphorylation of Sc in vivo modulates its susceptibility to E(spl) m7 repression. (A, C, E, G, I) Thoraces from pnr-Gal4 ﬂies overexpressing the indicated Sc variants. The pnr expression domain is boxed in panel A. Note the production of ectopic bristles by all Sc variants, except Sc[RQEQ], where mild bristle loss is seen (I). C (UAS-myc-sc⁸) and E (UAS-myc-sc[m3p]^7b) are pharate escapers. G (UAS-myc-sc[1–320]^9d) is the only viable line with pnr-Gal4 and I (UAS-myc-sc[RQEQ]^13a) is viable, as are all Sc[RQEQ] lines. (B, D, F, H, J) Thoraces from pnr-Gal4 ﬂies overexpressing the indicated Sc variants along with E(spl)m7. m7 strongly inhibits ectopic bristle production, with the exception of panel F, where a large number of bristles persist. (K) pnr-Gal4; UAS-m7. UAS-myc-sc[1–320]^64b; line 64b never gives pharate escapers when expressed alone (without m7) and produces many more SOPs in larvae than line 9d (G). Still it is more effectively suppressed by UAS-m7 than Sc[m3p] (F). (L) pnr-Gal4; UAS-m7. UAS-myc-sc[m5p]^73a; line 73a never gives pharate escapers when expressed alone (without m7) but is less susceptible than the equally inviable Sc[1–320]^64b to m7 overexpression. (M) myc-tagged proteins (as indicated) were expressed and immunoprecipitated from S2 cells. Immunoprecipitation efﬁciency (Myc, lower panel) and the presence of coprecipitated HA-tagged m7 (HA, middle panel) were assayed. Upper panel shows the levels of the HA-m7 in the cell extracts (input). All Sc variants interact with HA-m7. GFP, the negative control and DaΔTADs (lacks the AD1 and LH domains) do not immunoprecipitate HA-m7.