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. 2015 Feb 17;43(5):2767–2779. doi: 10.1093/nar/gkv087

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Total macroscopic kinetic rate (k_tot) from force-jump experiments. (a, b) DNA extension as a function of time after increasing the force from 2 pN to a preset value of: 15 pN (red), 25 pN (blue), 35 pN (purple), 45 pN (green), 55 pN (yellow). Relaxations are well described by a single-exponential function and allow us to obtain k_tot at the two concentrations investigated: 100 nM in panel (a) and 1 μM in panel (b). (c) Force dependence of k_tot at the two investigated concentrations. The intercalation rate increases exponentially with force with similar slopes for both concentrations.