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. 2015 Feb 20;43(5):2575–2589. doi: 10.1093/nar/gkv101

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Cdk12/CycK is present at the novel HR genes to promote phosphorylation of the CTD of RNAPII at Ser2. (A, B and C) Control (blue bars) or Cdk12 knockdown HeLa DR-GFP cells (red bars) were subjected to ChIP-qPCR analysis to determine the levels of Cdk12 (panel A), total/Ser5-P RNAPII (panel B) and Ser2-P RNAPII (panel C) occupancy at RAD51D intergenic region (IR) and three gene-specific regions as indicated below the graphs. The identity of genes analyzed is indicated on top of each graph. Levels of IgG signals at IR and gene regions are presented as gray bars. Where these levels were significantly lower than those obtained with specific antibody, the error bars were omitted for simplicity reasons. Results are presented as percent of input DNA and plotted as the mean ± SD.