Table 2. Thermodynamic parameters of the dissociation of the RNA duplexes determined by DSC.
| No. | Name | Tm [°C] for duplex [20 μM] | -ΔH ± SD [kcal/mol] | -ΔScal ± SD [cal/K mol] | -ΔG25 for duplex formation [kcal/mol] |
|---|---|---|---|---|---|
| 1 | U-A | 60.3 ± 0.3 | 111.6 ± 5.6 | 335.8 ± 16.6 | 15.1 ± 0.8 |
| 2 | S-A | 64.3 ± 0.1 | 116.4 ± 2.8 | 348.0 ± 8.6 | 16.5 ± 0.4 |
| 3 | H-A | 53.1 ± 0.4 | 96.0 ± 8.2 | 296.1 ± 25.2 | 11.0 ± 1.0 |
| 4 | U-G | 57.1 ± 0.3 | 106.8 ± 2.6 | 324.2 ± 7.5 | 13.7 ± 0.5 |
| 5 | S-G | 54.2 ± 0.2 | 91.0 ± 0.5 | 278.0 ± 1.4 | 11.1 ± 0.1 |
| 6 | H-G | 54.6 ± 0.3 | 76.1 ± 3.7 | 231.6 ± 10.7 | 9.6 ± 0.6 |
All values are derived as averages of three independent measurements.