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. 2015 Feb 24;43(5):2888–2901. doi: 10.1093/nar/gkv110

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Ribosome assembly on wild type and mutant HCV IRES. (A) Ribosome assembly on wild-type ³²P-labelled HCV IRES RNA in the presence of either cycloheximide or GMPPNP. (B–D) ³²P-labelled wild type and mutant HCV IRES RNAs were used as template for translation in RRL, loaded onto 10–30% sucrose gradient and centrifuged to separate the different translation complexes. Different fractions were collected and the radioactivity associated with each fraction was measured. Graph was plotted with % CPM against the respective fraction number.