Figure 5.

Interaction of RPS5 with HCV IRES and 3′ UTR. (A) Direct UV cross-linking of either α-³²P-labelled HCV IRES or 3′ UTR with increasing concentrations of 40S ribosomal subunit (5 and 10 pmol) This is a representative image of three independent experiments. (B) Competition UV cross-linking of α-³²P-labelled HCV IRES with 40S ribosomal subunit (10pmol) in the presence of either unlabelled HCV IRES RNA (75- and 150-fold) or 3′ UTR RNA (150-fold). This is a representative image of three independent experiments. (C) Direct UV cross-linking of α-³²P-labelled HCV IRES or 3′ UTR with increasing concentrations of recombinant ribosomal protein S5 (4 and 8 pmol). Lane number 6 corresponds to UV cross-linking of La protein with α-³²P-labelled 3′ UTR as a control for the integrity of the 3′ UTR probe. This is a representative image of three independent experiments.