Figure 1.

Sso MCM domain organization and amino acid sequence of the C-terminal region. Denomination of deletion mutants used here on top. WA: Walker A motif, WB: Walker B motif, S1: sensor 1 region, RF: arginine finger, S2: sensor 2 region. Asterisks represent point mutations in the Sso MCM C-terminal domain for the analysis of their role in controlling the Sso MCM ATPase and helicase activity. In addition, Sso MCM constructs were generated where the underlined stretches of amino acids were deleted either separately or both in combination, respectively. For a detailed list of constructs used in this study see Supplementary Table S6. Domain organization scheme adapted from (3).