Figure 2.

Protein-primed initiation of ϕ29 TP-DNA replication with wild-type or N-terminal mutant TPs either in the absence (A) or in the presence (B) of protein p6. The initiation assays were performed as described in Materials and Methods in the presence of 12 nM of TP, 12 nM of DNA polymerase and 1.6 nM of ϕ29 TP-DNA as template either in the absence or in the presence of 35 μM of protein p6. After incubation for 2 min at 30°C, samples were processed as described in Materials and Methods, analysed by SDS-PAGE and quantified by densitometric analysis of the autoradiographs. The figure is representative of three independent experiments. Closed arrows indicate the position of TP-dAMP and open arrows indicate the position of ΔNt TP-dAMP.