Skip to main content
. 2015 Feb 26;43(5):2790–2801. doi: 10.1093/nar/gkv127

Figure 3.

Figure 3.

Template-independent TP-deoxynucleotidylation with wild-type and N-terminal mutant TPs. (A) TP-dAMP formation with either wild-type or N-terminal mutant TPs in the absence of template. Reactions were carried out as described in Materials and Methods in the presence of 24 nM of TP and 24 nM of DNA polymerase for 1 h at 30°C. Closed arrow indicates the position of TP-dAMP and open arrow indicates the position of ΔNt TP-dAMP. (B) Competition for DNA polymerase between wild-type and N-terminal mutant TPs. Reactions were carried out as described for the template-independent TP-dAMP formation assay but adding increasing amounts of mutant TPs. TP-dAMP formation values indicated are relative to those in the absence of competition (100%). As a control, S232C mutant TP was used. Data are represented as mean and standard deviation derived from three independent experiments.