Figure 4.

Transition assay with wild-type and N-terminal mutant TPs either in the absence or in the presence of protein p6. Reactions were carried out as described in Materials and Methods. Twelve nM of TP were incubated with 12 nM of DNA polymerase mutant N62D in the presence of 5 μM dATP, dTTP and dGTP (missing dCTP). Samples were incubated at 30°C for 5 min either in the absence or in the presence of 35 μM of protein p6. The position of TP-dAMP initiation and TP-dNMP abortive products are indicated. The figure is representative of three independent experiments. A schematic representation of the two origins of replication and the last products expected from reactions initiating from both ends are depicted on top of the figure.