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. 2015 Feb 26;43(5):2790–2801. doi: 10.1093/nar/gkv127

Figure 7.

Figure 7.

Amplification with TP mutant ΔNt is recovered upon addition of wild-type TP/DNA polymerase heterodimer but not upon addition of TP ΔNt/DNA polymerase heterodimer. The upper panel shows the amplification reactions with TP mutant ΔNt at the indicated times and the amplification reactions after the addition of either wild-type TP/DNA polymerase heterodimer or TP ΔNt/DNA polymerase heterodimer at 90 min post-incubation. The position of unit length ϕ29 DNA is indicated. The figure is representative of three independent experiments. The bar graph shows the kinetics of DNA synthesis in ng for each of the above-mentioned conditions at the indicated times. Bars represent mean and standard deviation derived from three independent experiments. The bottom panel shows the amplification factors calculated as the ratio between the amount of DNA in ng at the end of the reaction (input DNA plus synthesised DNA) and the amount of input DNA.