Table 1. Activity of wild-type and N-terminal mutant TPs.
| TP-dAMP formationa | TP-DNA replicationa | TP-DNA amplification | |||||
|---|---|---|---|---|---|---|---|
| TP-DNA | No template | ||||||
| TP | − p6 | + p6 | − p6 | + p6 | %b | AFc | |
| wt TP | 100 | 100 | 100 | 100 | 100 | 100 | 137 ± 22 |
| R19A | 75 ± 13 | 102 ± 10 | 98 ± 27 | 44 ± 9 | 93 ± 8 | 110 ± 8 | 149 ± 14 |
| K25A | 108 ± 17 | 115 ± 7 | 87 ± 5 | 89 ± 3 | 98 ± 2 | 121 ± 6 | 165 ± 27 |
| K27A | 21 ± 4 | 108 ± 7 | 53 ± 5 | 3 ± 1 | 73 ± 9 | 12 ± 3 | 18 ± 6 |
| K25A/K27A | 19 ± 4 | 116 ± 9 | 58 ± 12 | 3 ± 1 | 73 ± 5 | 13 ± 4 | 19 ± 8 |
| K32A/K33A/K34A | 40 ± 17 | 111 ± 11 | 99 ± 12 | 16 ± 5 | 97 ± 6 | 104 ± 15 | 140 ± 9 |
| ΔNt | 10 ± 4 | 109 ± 12 | 27 ± 4 | 1 ± 0.3 | 60 ± 5 | 3 ± 2 | 5 ± 3 |
aNumbers indicate the average percentage of activity of the mutant TPs relative to the wild-type TP and the standard deviation obtained from three independent experiments.
b% indicates the average percentage of activity of the mutant TPs relative to the wild-type TP and the standard deviation obtained from three independent experiments at 60 min of incubation.
cAF indicates the average amplification factor of the mutant TPs relative to the wild-type TP and the standard deviation obtained from three independent experiments at 60 min of incubation.