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. 2015 Feb 26;43(5):2790–2801. doi: 10.1093/nar/gkv127

Table 1. Activity of wild-type and N-terminal mutant TPs.

TP-dAMP formationa TP-DNA replicationa TP-DNA amplification
TP-DNA No template
TP − p6 + p6 − p6 + p6 %b AFc
wt TP 100 100 100 100 100 100 137 ± 22
R19A 75 ± 13 102 ± 10 98 ± 27 44 ± 9 93 ± 8 110 ± 8 149 ± 14
K25A 108 ± 17 115 ± 7 87 ± 5 89 ± 3 98 ± 2 121 ± 6 165 ± 27
K27A 21 ± 4 108 ± 7 53 ± 5 3 ± 1 73 ± 9 12 ± 3 18 ± 6
K25A/K27A 19 ± 4 116 ± 9 58 ± 12 3 ± 1 73 ± 5 13 ± 4 19 ± 8
K32A/K33A/K34A 40 ± 17 111 ± 11 99 ± 12 16 ± 5 97 ± 6 104 ± 15 140 ± 9
ΔNt 10 ± 4 109 ± 12 27 ± 4 1 ± 0.3 60 ± 5 3 ± 2 5 ± 3

aNumbers indicate the average percentage of activity of the mutant TPs relative to the wild-type TP and the standard deviation obtained from three independent experiments.

b% indicates the average percentage of activity of the mutant TPs relative to the wild-type TP and the standard deviation obtained from three independent experiments at 60 min of incubation.

cAF indicates the average amplification factor of the mutant TPs relative to the wild-type TP and the standard deviation obtained from three independent experiments at 60 min of incubation.