. 2015 Feb 26;43(5):2790–2801. doi: 10.1093/nar/gkv127

Table 2. Nucleotide binding affinity of the DNA polymerase with TP N-terminal mutants either in the absence or in the presence of protein p6.

	K_m^a (μM)
TP	− p6	+ p6
wt TP	43.5 ± 5.5	4.6 ± 1.5
K25A	33.4 ± 4	4.4 ± 0.7
K27A	354.4 ± 75	2.7 ± 1.4
K25A/K27A	512.3 ± 40	2.7 ± 0.1

^aMichaelis constant values (mean and standard deviation) of the DNA polymerase for dATP in template-directed initiation reactions using the indicated TP as primer, either in the absence or in the presence of protein p6. Values are derived from at least three independent experiments.