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. 2015 Feb 26;43(5):2625–2637. doi: 10.1093/nar/gkv133

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Rat1/Rai1 does not have ATPase activity. (A) ATPase activity assay of Rat1/Rai1. Calf intestinal phosphatase (CIP), SV40 T antigen (T ag) and Xrn1 were used as controls. Highly purified Rat1/Rai1 fractions from size-exclusion chromatography (Superose 6) show no ATPase activity. (B) Quantification of the remaining RNAs from the in vitro transcription termination assay with various non-hydrolyzable ATP analogs.