Figure 4.

Other 5′-3′ exoribonucleases and Escherichia coli RNAP. In vitro assays were performed using EC1 scaffold. (A) ATP enhances RNAPII termination by recombinant hXrn2 in vitro. Quantification of the remaining RNAs is shown below. (B) Yeast Xrn1, Rat1's cytoplasmic counterpart, terminates RNAPII regardless of the presence of ATP in vitro. Quantification of the remaining RNAs is shown below. (C) Rat1/Rai1 cannot terminate E. coli RNAP in vitro and addition of each NTP had no effect either. Representative gel image is presented on the left and quantification of the remaining RNA is presented in the right panel.