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. 2015 Feb 26;43(5):2625–2637. doi: 10.1093/nar/gkv133

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Exoribonucleolytic-deficient rat1 (rat1EDD) cannot terminate RNAPII and Rtt103 does not rescue the termination defect of rat1EDD. (A) (Left) rat1EDD/Rai1, regardless of Rtt103 addition, does not terminate RNAPII. (Right) Quantification of the extended run-off RNA amounts relative to the initial starting RNAs without or with ATP addition. rat1EDD does not reduce the RNA elongation efficiency by RNAPII compared with the no nuclease control. (B) Multiple copies of the Rtt103 gene cannot rescue the lethality of the rat1EDD mutation. (C) Elution profiles of size-exclusion chromatography (Superose 6) of Rat1/Rai1/Rtt103 (RRR) and rat1EDD/Rai1/Rtt103 (rRR) show that most of the Rat1/Rai1 is co-eluted with Rtt103, whereas most of rat1EDD/Rai1 is not. These profiles indicate that rat1EDD shows reduced binding to Rtt103 compared with wild-type Rat1.