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. 2015 Feb 26;43(5):2638–2654. doi: 10.1093/nar/gkv138

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — TRIM32, Ago2 and DDX6 directly interact with each other in vivo. (a) Immunoblots of IPs from E10.5 to E14.5 embryonic brain lysates using antibodies against DDX6 or GFP (negative control). DDX6, TRIM32 and Ago2 were detected with the indicated specific antibodies in the IP and lysate. (b) Immunoblots of IPs from adult brain lysates using antibodies against DDX6, Ago2 or GFP (negative control). DDX6, TRIM32 and Ago2 were detected with the indicated specific antibodies in the IP and lysate. Abbreviations: EGFP, enhanced green fluorescent protein; Ago, Argonaute; IP, immunoprecipitation; WB, western blot; Neg. Ctrl., negative control.