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. 2015 Feb 26;43(5):2638–2654. doi: 10.1093/nar/gkv138

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — TRIM32, Ago2 and DDX6 colocalize in NSCs and neurons. (a and b) Immunostaining of neurospheres derived from TRIM32 +/+ (a) and TRIM32 −/− (b) mice, cultured under maintenance conditions and 5 days after the induction of neuronal differentiation, with the indicated markers. Single optical planes are shown. The right panels show a higher magnification of the boxed areas. Scale bars = 20 μm; for high magnifications, 5 μm. (c–e) Diagrams showing Pearson's coefficients for the colocalization of TRIM32 and Ago2 (c), TRIM32 and DDX6 (d) and Ago2 and DDX6 (e) in NSCs and neurons. Colocalization was defined as a Pearson's coefficient ≥0.3 (blue dotted line) (mean ± SEM; n ≥ 20 cells, N = 3 independent TRIM32 +/+ and TRIM32 −/− neurosphere cultures).