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. 2015 Feb 26;43(5):2638–2654. doi: 10.1093/nar/gkv138

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — DDX6 is sufficient to induce neuronal differentiation in NSCs. (a) TRIM32 +/+ and −/− neurospheres were nucleofected with EGFP-FLAG or DDX6-Venus-FLAG and differentiated into neurons for 5 days. Immunostaining of the cells labeled as indicated is shown. Overexpressed DDX6 shows a dotted cytoplasmic localization. Scale bar = 15 μm. (b and c) Diagram showing the percentage of nucleofected TRIM32 +/+ and −/− neurons that are positive for the young neuronal marker TuJ1 (b) or the proliferation marker Ki67 (c) (mean ± SEM; n ≥ 160 cells, N = 4 independent TRIM32 +/+ and −/− neurosphere cultures; t-test, **P < 0.01, ***P < 0.005).