Figure 5.

Ku70 and Ku80 inhibit ASO activity. (A) siRNA mediated reduction of Ku70 or Ku80 proteins. siRNA targeting LRPPRC was used as a negative control. Whole cell lysates from control cells or cells treated with siRNAs for 36 h were subjected to western analyses for Ku70 or Ku80 protein. γ-tubulin served as a loading control. (B, C) Reduction of Ku70 or Ku80 protein, but not LRPPRC, increased the activity of ASOs targeting (B) PTEN and (C) NCL1 mRNAs as detected by qRT-PCR. (D) Reduction of Ku70, TCP1β or LRPPRC mRNAs by siRNA treatment was demonstrated by qRT-PCR. (E) qRT-PCR analysis for ASO-directed PTEN mRNA reduction in different test cells. Where shown, the error bars are standard deviations of three independent experiments. P-values were calculated based on unpaired t-test (n = 3), and the significance is indicated above the bars. ‘NS’: not significant. ‘*’: 0.01<P<0.05; ‘**’: 0.001<P<0.01; ‘***’: 0.0001<P<0.001; and ‘****’: 0.00001<P<0.0001.