Figure 6.

DNA lesion removal from the sense strand of the 5′-ends (TSS) and 3′-ends (END) of the genes ATR, PAPPA, SLIT2, ANXA2 and DRAM1. TC-NER deficient XP-C cells were irradiated with 10 J/m² of UVC light and lesion removal estimated using qPCR. Note that lesions are repaired slower from the 3′-end compared to the 5′-end of long genes and similarly in the SLIT2 gene that showed no RNA synthesis recovery. Remaining DNA lesions are expressed as lesions/10 kb of the sense strand as calculated assuming a Poisson distribution (see Materials and Methods). The values represents the mean of quadruplicate samples from two independent biological experiments with bars representing the standard deviation. Sense strand normalization was related to DNA repair obtained from non-transcribed genes. Statistics with Two-way ANOVA: ‘a’ is significantly different from ‘b’.