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. 2013 Sep 28;11(5):304–311. doi: 10.1016/j.gpb.2013.08.002

Figure 2.

Figure 2

UCB-iPSCs express pluripotency-specific markers A. Morphology of UCB-iPSCs. Scale bar, 200 μm. B. UCB-iPSCs express alkaline phosphatase. Feeder cells were used as negative control. Scale bar is 200 μm. C. Karyotyping of UCB-iPSCs. D. mRNA expression in UCB-iPSC lines performed by RT-PCR. UCB-iPSCs express pluripotency markers Pou5f1 (Oct4), Sox2, Nanog, Klf4, Rex1, Lin28a and Lin28b. Water was used as a negative control, whereas H9 hESCs were used as a positive control. E. Immunofluorescence results of UCB-iPSCs. UCB-iPSCs express pluripotency markers OCT4, SOX2, NANOG, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81 at the protein level. SSEA: stage-specific embryonic antigen. Scale bar, 100 μm.