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. 2015 Mar 13;5:9092. doi: 10.1038/srep09092

Figure 4. Onset of tubular organic cation uptake in developing kidneys in culture and in vivo.

Figure 4

Specific uptake of the fluorescent cation DAPI with or without the Oct inhibitors metformin and cimetidine was investigated in E11.5 mouse kidneys grown in Sebinger culture after 3 days (A), 4 days (B) or 6 days (C) in culture. Tubules were also labelled by uptake of 6-CF (A–C, bottom panels). Cultures in (A) and (B) were imaged using an epifluorescence microscope. Cultures in (C) were imaged using a confocal microscope and images shown are of a single confocal plane. Scale bars represent 500 μm. D) Total mRNA was extracted from kidneys at various embryonic stages and from adult murine kidney. Detection of mRNA transcripts from two renal murine organic cation transporters was evaluated by multiplex RT-PCR (gene of interest and β-actin). (E) Total mRNA was extracted from E11.5 embryonic kidneys cultured for 1, 2, 3, 4, or 6 days. Detection of mRNA transcripts from two murine renal organic cation transporters was evaluated by multiplex RT-PCR (gene of interest and β-actin).