Figure 5. DAPI staining outside of tubules shows necrotic or apoptotic nuclei.

Uptake of DAPI by Oct family cation transporters after 6 days in Sebinger culture either without (A) or with (B) Oct inhibitors metformin and cimetidine followed by staining with Cy5-conjugated Annexin-V. Annexin-V staining is associated with DAPI-positive apoptotic nuclei outside the tubules (A(i)) and B(i), and boxed enlargements white arrows), but not with DAPI-positive (non-apoptotic) tubular nuclei (Ai). Images were captured by confocal microscopy. Scale bars represent 500 μm (A, B), or 100 μm (A(i), B(i)).