. 2015 Mar 12;81(7):2625–2634. doi: 10.1128/AEM.04033-14

TABLE 3.

Isobutene formation rates for PtM3K and ScMVD^a

Enzyme	Isobutene formation rate
	Whole cells (pmol · min⁻¹ · g cells⁻¹)	Crude extract (pmol · min⁻¹ · mg total protein⁻¹)	Purified protein (pmol · min⁻¹ · mg protein⁻¹)
	Whole cells (pmol · min⁻¹ · g cells⁻¹)	Crude extract (pmol · min⁻¹ · mg total protein⁻¹)	30°C	50°C
PtM3K	507 ± 137	261 ± 19	26 ± 2	2,880 ± 140
ScMVD	7.4 ± 1.5	1.6 ± 0.3	2.3 ± 1.2	NA

The concentration of 3-HIV used was 50 mM. For the crude extract and purified enzyme experiments, 40 mM ATP was added. Assays were done in triplicate, and the means ± standard errors of the means for formation rates are shown. Isobutene was quantified by GC-MS analysis of the headspace. Due to the very low aqueous solubility of isobutene (51), its concentration in the liquid phase was considered negligible, and it was not taken into consideration. NA, not available.