FIG 3.

The BCAA auxotrophy of KGMA5511 (ΔpyrE ΔilvC::pyrE). (A) BCAA auxotrophy of strain KGMA5511. Strains KGMA0119 (wild type) and KGMA5511 were streaked onto a minimal agar plate lacking (upper panel) or containing (lower panel) 12 mM BCAAs. (B) Growth profiles of KGMA0119 and KGMA5511 strains. KGMA0119 was cultured in YPD broth lacking BCAAs, and KGMA5511 was cultured in YPD broth lacking or containing various concentrations of BCAAs. White triangles, KGMA0119 without BCAAs; white squares, KGMA5511 without BCAAs; light gray squares, KGMA5511 with 0.5 mM BCAAs; dark gray squares, KGMA5511 with 4 mM BCAAs; black squares, KGMA5511 with 12 mM BCAAs. Experiments were conducted in triplicate, and error bars indicate standard deviations.