TABLE 1.

Host range of phage PBC1 and lytic activity of LysPBC1 and its enzymatic active domain LysPBC1_EAD

Species	Strain no.a	Plaque (PBC1)	Relative lytic activityb
			LysPBC1	LysPBC1_EAD
B. cereus group strains
B. cereus	ATCC 27348	−	+++	+
	ATCC 21768	+	+++	+
	ATCC 13061	−	+++	+
	ATCC 14579	−	++	+
	ATCC 21772	−	+++	++
	ATCC 10876	−	++	+
	KCTC 3674	−	++	+
	ATCC 10987	−	+++	+
	KCTC 1094	−	++	−
	NCCP 10623	−	++	+
	NCCP 10624	−	+++	+
	NCCP 10634	−	+	+
	NCCP 10715	−	++	−
	NCCP 10841	−	++	+
	NCCP 10856	−	+	−
	NCCP 11306	−	++	+
	NCCP 11308	−	+++	++
	NCCP 11309	−	++	+
	NCCP 11311	−	++	+
	NCCP 12448	−	+++	+
	NCCP 14043	−	++	++
	NCCP 14796	−	++	+
B. thuringiensis	ATCC 10792	−	++	+
B. mycoides	ATCC 6462	−	++	+
B. weihenstephanensis	KCTC 3975	−	+	−
Other Gram-positive strains
B. megaterium	JCM 2506	−	+	+
B. subtilis	ATCC 6051	−	+	++
	ATCC 6633	−	+	++
	ATCC 23857	−	+	++
	JCM 2508	−	−	+
B. licheniformis	JCM 2505	−	+	++
Bacillus sphaericus	JCM 2502	−	+	+
Bacillus circulans	JCM 2504	−	+	+
L. monocytogenes	Scott A	−	−	−
Listeria innocua	ATCC 33090	−	−	−
Clostridium perfringens	ATCC 3624	−	−	−
Clostridium indolis	ATCC 25771	−	−	−
Staphylococcus aureus	ATCC 29213	−	−	−
Enterococcus faecalis	ATCC 29212	−	−	−
Gram-negative strains
E. coli	MG1655	−	++	++
Shigella flexneri	2a strain 2457T	−	++	+++
Cronobacter sakazakii	ATCC 29544	−	++	++

^aATCC, American Type Culture Collection; KCTC, Korean Collection for Type Cultures; NCCP, National Culture Collection for Pathogens; JCM, Japan Collection of Microorganisms.

^bGram-negative bacteria were pretreated with EDTA. The relative lytic activity of endolysin was obtained by measuring the percent drop in OD₆₀₀ in 8 min. −, no lysis; +, limited lysis; ++, medium lysis; +++, rapid lysis.