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Anthranilate enhances static biofilm formation. Static biofilm assay was carried out as described in Materials and Methods. Wild-type PAO1 cells were grown in 96-well plates and the measurement was taken at 24 h after inoculation. -, DMSO as a buffer control; IN, 0.4 mM indole; AA, 0.1 mM anthranilate; AA + IN, 0.1 mM anthranilate plus 0.4 mM indole. *, P < 0.05; ***, P < 0.005.
