FIG 8.

Anthranilate effects on the levels of c-di-GMP and expression of EPS biosynthetic genes. (A) P. aeruginosa cells harboring pSKcdrA (cdrA-lacZ fusion) were grown without (−) or with 0.1 mM anthranilate for 20 h, and β-galactosidase activity, which reflects the c-di-GMP level, was measured. For comparison, the reporter cells were grown with various concentrations of SNP under the same condition. The c-di-GMP level of nontreated cells was set to 100%, and the relative c-di-GMP levels of treated cells are presented from β-galactosidase activity. (B) P. aeruginosa cells were cultivated with or without 0.1 mM anthranilate, and the transcripts of Psl, Pel, and alginate biosynthetic operons were measured by quantitative real-time PCR analysis. The transcription levels are presented relative to the transcription level of nontreated cells. ***, P < 0.005.