TABLE 1.
Bacterial strains and plasmids used in this study
| Name | Descriptiona | Reference |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Wild-type P. aeruginosa | 43 |
| MW1 | lasI rhlI double mutant of PAO1 | 44 |
| E. coli strain DH5α | supE44 ΔlacU169 (ф80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 45 |
| Plasmids | ||
| pAB1 | gfp-mut2 gene in pMF54; Apr | 46 |
| pQF50 | Broad-host-range lacZ fusion plasmid; Apr | 29 |
| pJN105 | araC-pBAD cassette cloned in pBBR1MCS-5; Gmr | 47 |
| pJN105A | Plasmid for AntR overexpression; antR ORF in pJN105; Gmr | 23 |
| pSC11 | lasIp-lacZ reporter in pQF50; Apr | 48 |
| pJL101 | PA1897p-lacZ reporter in pQF50; Apr | 24 |
| pJL201 | antAp-lacZ fusion in pQF50; Apr | 23 |
| pSKcdrA | cdrA-lacZ fusion in pQF50; Apr | This study |
a
Gmr, gentamicin-resistance; Apr, ampicillin and carbenicillin resistance; ORF, open reading frame.