TABLE 3.
Genetic engineering of S. epidermidis using phage Φ187-mediated plasmid transfer
| Bacterial strain | Counterselection efficiency (%)a | sigB mutagenesis efficiency (%)b |
|---|---|---|
| S. epidermidis 33b | 100 | 85.7 |
| S. epidermidis IVK83 | 96.4 | 42.8 |
a
Counterselection efficiency was calculated after anhydrotetracycline selection by dividing the number of chloramphenicol-sensitive clones by the total number of tested isolates.
b
Mutagenesis efficiency was calculated after PCR analysis by dividing the number of sigB deletion mutants by the total number of tested isolates.