TABLE 1.
Oligonucleotide probes used for fluorescence in situ hybridization of rumen ciliates in this study
| Name of probe | Fluorescent dyea | 5′–3′ sequence | Reference or source |
|---|---|---|---|
| CHA1350 | Cy3 | TGA ATA TTC ACC GAG TGA ATA CCA | This study |
| EUKb1193 | Cy5 | GGG CAT MAC DGA CCT GTT | 23 |
| NON1350b | Cy5 | ACT TAT AAG TGG CTC ACT TAT GGT | This study |
a
Probes were fluorescently labeled using cyanine 3 (Cy3) and cyanine 5 (Cy5). The optimal formamide concentration for a hybridization temperature of 46°C was 25 to 30%.
b
The nonsense probe was designed as the reverse of the Charonina ventriculi-specific probe and served as a negative control.