TABLE 2.
Artificial gfp genes, plasmids, host strains, cell lines, phages, and virus used in this study
| Gene, plasmid, host strain, cell line, phage or virus | Accession no. | Description |
|---|---|---|
| Genes | ||
| gfp | JC175864 | The original gfpmut3 gene (2) |
| lgfp | LC018331 | The gfpmut3 gene with all codons changed to those with the lowest usage in E. coli |
| lgfpΔ1 | LC018332 | The modified lgfp gene with 180 bp (25%) deleted from C terminus |
| lgfpΔ2 | LC018333 | The modified lgfp gene with 357 bp (50%) deleted from C terminus |
| lgfpΔ3 | LC018334 | The modified lgfp gene with 537 bp (75%) deleted from C terminus |
| hgfp | LC018335 | The gfpmut3 gene with all codons changed to those with the highest usage in E. coli |
| hgfpΔ1 | LC018336 | The modified hgfp gene with 180 bp (25%) deleted from C terminus |
| hu-lgfp | LC018337 | The AcGFP1 gene with all codons changed to those with the lowest usage in H. sapiens |
| Plasmids | ||
| pLGFP | LC018338 | IPTG-inducible lgfp, ColE1 origin, Ampr |
| pHGFP | LC018344 | IPTG-inducible hgfp, ColE1 origin, Ampr |
| pLGFPm | LC018342 | IPTG-inducible lgfp, p15A origin, Ampr |
| pLGFPl | LC018342 | IPTG-inducible lgfp, SC101 origin, Ampr |
| pLGFPΔ1 | LC018339 | IPTG-inducible lgfpΔ1, ColE1 origin, Ampr |
| pLGFPΔ2 | LC018340 | IPTG-inducible lgfpΔ2, ColE1 origin, Ampr |
| pLGFPΔ3 | LC018341 | IPTG-inducible lgfpΔ3, ColE1 origin, Ampr |
| pLΔNG | LC018348 | IPTG-inducible lgfpΔ1, aTc-inducible gfp, ColE1 origin, Ampr |
| pHΔNG | LC018349 | IPTG-inducible hgfpΔ1, aTc-inducible gfp, ColE1 origin, Ampr |
| pPAL | LC018350 | f1 phage infection-inducible lgfp, ColE1 origin, Ampr |
| pPAH | LC018351 | f1 phage infection-inducible hgfp, ColE1 origin, Ampr |
| pRARE | NAa | Carries rare-codon tRNAs, p15A origin, chloramphenicol resistance |
| pYEG | LC018345 | Galactose-inducible yEGFP in S. cerevisiae, aureobasidin A resistance, CEN/ARS |
| pYLGFP1 | LC018346 | Galactose-inducible lgfp in S. cerevisiae, aureobasidin A resistance, CEN/ARS |
| pYLGFP2 | LC018347 | Galactose-inducible hgfp in S. cerevisiae, aureobasidin A resistance, CEN/ARS |
| pTRE-Luc | NA | Dox-inducible luciferase in mammalian cells, G418 resistance |
| pTRE-G1 | LC018352 | Dox-inducible hu-lgfp in mammalian cells, G418 resistance |
| Host strains | ||
| E. coli | ||
| Mach1 | F− ϕ80(lacZ)ΔM15 ΔlacX74 hsdR(rK− mK+) ΔrecA1398 endA1 tonA | |
| XL-10 | deoR endA1 gyrA96 hsdR17(rK− mK+) recA1 relA1 supE44 thi-1 Δ(lacZYA-argF)U169 ϕ80dlacZΔM15 F− λ− PN25/tetR placIq/lacI | |
| JM2.300 | lacI22 LAM e14 rpsL135 (Strr) xyl-7 mtl-1 thi-1 | |
| AK4 | λ− lacI::Km mutant of K-12 (CGSC7296) wild-type strain | |
| S. cerevisiae YPH499 | MATα his3-Δ200 leu2-Δ1 lys2-801 trp1-Δ1 ade2-101 ura3-52 | |
| Human cell lines | ||
| HeLa-Tet-ON | HeLa cells optimized for Tet-ON gene expression system | |
| MCF7-Tet-ON | MCF7 cells optimized for Tet-ON gene expression system | |
| HEK293-Tet-ON | HEK293 cells optimized for Tet-ON gene expression system, host of recombinant adenovirus | |
| Phages | ||
| T4 | Enterobacterial phage T4, linear double-stranded DNA phage | |
| T7 | Bacteriophage T7, linear double-stranded DNA phage | |
| λ | Enterobacterial phage l, linear double-stranded DNA temperate phage | |
| f1 | Enterobacterial phage f1, a filamentous single-stranded DNA phage | |
| MS2 | Bacteriophage MS2, an icosahedral linear single-stranded RNA+ phage | |
| Recombinant adenovirus | A recombinant virus vector for gene transfer, no gene was coded in this expt |
a
NA, not available.