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Amino acid alignment of Cthe_0345 (MDH) and Cthe_1053 (LDH) from C. thermocellum and b3236 (MDH) from E. coli K-12 MG1655. Shaded areas indicate positions 18, 86, 90, 212, and 216 based on the amino acid positions of Cthe_0345 (MDH).