TABLE 1.

Primers used for gap analysis to determine whether transcription of all of the genes in the operon occurred

Gap (genes)	Primer sequence (5′–3′)
	Forward	Reverse
Gap BC (flgB and flgC)	TCGTCCACGGCGAAGATCAGGT	TATGGCCCGCCGCCTATGGT
Gap CE (fligC and fliE)	GACCTCGCAGCGCGGGTATC	TCCACTCTCGGTTCCGGCGT
Gap EF (fliE and fliF)	AGTGATGGAAGCGTACCGCGAAC	CGACCACAGTCAGGCCGACG
Gap FG (fliF and fliG)	CACCGGCGAAGAGCGTCTGG	GGCACGGTGTCCAGTTCGACC
Gap GH (fliG and fliH)	GCGCGGCCACCATGATCAGA	GGGCTTCTTCACGCAGCCGT
Gap HI (fliH and fliI)	ACACCCCCGCGGTGGAGGATA	GGCGCCCAGAGGCGCTTTTA
Gap RB (fliR and flhB)	GCAGATGAACCTGCTGATGA	GCCATGGTCACAAAGGTTTT
Gap AB (flhB and flhA)	GCCTTGTATAGGCAGGTGGA	GGTAACCAGCAGAAGCGAAG
Gap AF (flhA and flhF)	CAGCTGCTCCAGAGGTTTCT	GCCATGATGTGCCCTTTTAT