TABLE 3.

Overview of all samples positive for Shiga toxin-producing E. coli, showing the results for all genes detected by PCR, the serotype, the result of culture confirmation, and the enumeration of generic E. coli CFU

Date	Producer	Sample	GeneDisc (PCR) result							Culture confirmation	Generic E. coli (CFU/100 ml or g)f
			stx1	stx2	eae	aggR	Serotype(s) among the top 5	eae variant(s)a	Serotype(s) with corresponding eae varianta
15 May 2012	2	Waterb	−	+	+	−	O145, O26	θ	−	−	6.0 × 101
15 May 2012	2	Waterb	−	+	+	−	O145, O26	θ	−	−	5.8 × 101
31 May 2012	2	Waterb	−	+	+	−	O26, O103, O145	β, θ	O26, O103	−	1.4 × 104
6 June 2012	2	Waterb	−	+	+	−	O26, O103, O145	β, θ, ε	O26, O103	−	1.1 × 103
18 June 2012	2	Waterb	−	+	+	−	O26, O103, O145	β	O26	−	1.2 × 103
4 July 2012	1	Waterb	−	+	+	−	O145, O26	−	−	−	2.1 × 101
14 September 2012	1	Waterb	+	−	+	−	O26, O103, O145	β, θ, ε, ϒ	O26, O103, O145	+ (O26)	1.7 × 102
14 September 2012	1	Waterc	+	−	+	−	O26, O145	β, ε, ϒ	O26, O145	+ (O26)	1.6 × 102
14 September 2012	1	Waterc	+	−	+	−	O26, O103, O145	β, θ, ϒ	O26, O103, O145	−	1.6 × 102
14 September 2012	1	Waterb	+	−	+	−	O26, O145	β, ε, ϒ	O26, O145	−	1.9 × 102
8 October 2012	4	Waterc	+	+	+	−	O26, O103, O145	β, θ, ε	O26, O103	−	1.6 × 102
14 September 2012	1	Substrate	+	−	+	−	O26, O103, O145	β	O26	+ (O26)	2.0 × 101
14 September 2012	1	Substrate	+	+	+	−	O26, O145	β, ϒ	O26, O145	+ (O26)	2.0 × 101
15 May 2013	1	Waterc	+	−	+	−	O26, O145	β	O26	−	1.5 × 102
15 May 2013	1	Waterb	+	−	+	−	O26, O145	β, θ	O26	−	8.6 × 101
21 August 2013	1	Waterc	+	−	+	−	−	−	−	−	1.1 × 101
18 September 2013	1	Waterb	−	+	+	−	O103, O145	β, θ	O103	−	2.1 × 102
15 October 2013	1	Waterc	−	+	+	−	O26, O103, O145	β, θ, ϒ	O26, O103, O145	−	1.9 × 103
15 October 2013	1	Waterb	−	+	+	−	O26, O145	β, ϒ	O26, O145	−	2.3 × 102
15 October 2013	1	Waterb	−	+	+	−	O26, O145	β, ϒ	O26, O145	−	2.2 × 102
15 May 2013	1	Substrate	+	−	+	−	O26	−	−	−	1.1 × 101
29 May 2013	1	Geese feces	+	+	+	−	−	−	−	−	NDg
15 May 2013	1	Cattle fecesd	+	+	+	−	O26, O145	β, θ	O26	−	ND
15 May 2013	1	Cattle fecese	+	+	+	−	O26, O145	β, θ	O26	−	ND
29 May 2013	1	Cattle fecesd	+	+	+	−	O26, O145	β, θ	O26	−	ND
29 May 2013	1	Cattle fecese	+	+	+	−	O26, O145, O103, O111	β, θ, ε	O26, O103, O111	−	ND
12 June 2013	1	Cattle fecesd	+	+	+	−	O145	θ, β	−	−	ND
12 June 2013	1	Cattle fecese	+	+	+	−	O26, O145, O103	β, θ, ϒ	O26, O103, O145	−	ND
26 June 2013	1	Cattle fecesd	+	+	+	−	O145, O103	θ, ε	O103	−	ND
26 June 2013	1	Cattle fecese	+	+	+	−	O26, O145, O103	β, θ, ε, ϒ	O26, O103, O145	−	ND
8 July 2013	1	Cattle fecesd	+	+	+	−	O145, O103	β, θ, ε	O103	−	ND
8 July 2013	1	Cattle fecese	+	+	+	−	O145, O103	β, θ, ε, ϒ	O103, O145	−	ND
24 July 2013	1	Cattle fecesd	+	+	+	−	O145, O103	β, θ, ε	O103	−	ND
24 July 2013	1	Cattle fecese	+	+	+	−	O145, O103	β, θ, ε, ϒ	O103, O145	−	ND
7 August 2013	1	Cattle fecesd	+	+	+	−	O145, O103	β, θ, ε	O103	−	ND
7 August 2013	1	Cattle fecese	+	+	+	−	O145, O103	β, θ, ε, ϒ	O103, O145	−	ND
21 August 2013	1	Cattle fecesd	+	+	+	−	O145, O103	β, θ, ε	O103	−	ND
21 August 2013	1	Cattle fecese	+	+	+	−	O145, O103	β, θ, ε, ϒ	O103, O145	−	ND
4 September 2013	1	Cattle fecesd	+	+	+	−	O145, O103	β, θ, ε	O103	−	ND
4 September 2013	1	Cattle fecese	+	+	+	−	O26, O145, O103	β, θ, ε, ϒ	O26, O103, O145	−	ND
18 September 2013	1	Cattle fecesd	+	+	+	−	O145, O103	θ, ϒ	O103, O145	−	ND
18 September 2013	1	Cattle fecese	+	+	+	−	O145, O103	β, θ, ε	O103	−	ND
1 October 2013	1	Cattle fecesd	+	+	+	−	O145, O103	β, θ, ϒ	O103, O145	−	ND
1 October 2013	1	Cattle fecese	+	+	+	−	O145, O103	β, θ, ε	O103	−	ND
15 October 2013	1	Cattle fecesd	+	+	+	−	O145, O103	β, ε, ϒ	O103, O145	−	ND
15 October 2013	1	Cattle fecese	+	+	+	−	O145, O103	β, θ, ε	O103	−	ND

^aData are from Bibbal et al. (20).

^bIrrigation point.

^cSource.

^dFeces from cattle >10 months old.

^eFeces from cattle <10 months old.

^fData correspond to the matrix: water (CFU/100 ml) or substrate (CFU/g).

^gND, not determined.