FIG 3.

Dictyostelium discoideum lysis induced by fluorescent pseudomonads strains harboring T3SS genes and biosurfactant production. Dictyostelium discoideum growth inhibition was calculated as described in Materials and Methods with bacteria grown at 25 or 37°C. Data are mean values from three independent experiments, and standard deviations are shown. Bacterial biosurfactant production was estimated by the drop collapse method as described in Materials and Methods. B+ indicates drop collapse and therefore biosurfactant production. nd, not determined (strains did not grow at 37°C).