FIG 4.

Symptoms in plant induced by fluorescent pseudomonad strains harboring T3SS genes. Induction of soft rot by P. syringae DC3000 at 25°C (A) and necrosis at 37°C by P. aeruginosa PAO1 (B) in chicory leaves (Cichorium intybus). Leaf panels were infiltrated in the middle vein with cells of each Pseudomonas strain at a concentration of 10⁸ CFU/ml in 0.9% (wt/vol) NaCl. Pictures were taken 72 h after infiltration and are representative of at least three independent experiments. Elicitation of a hypersensitive response in tobacco leaves (Nicotiana tabacum cv. Xanthi) at 25°C by P. syringae DC3000 (C) and P. fluorescens C7R12 (D). Leaf panels were infiltrated with cells of each Pseudomonas strain at a concentration of 2 × 10⁹ CFU/ml in 0.9% (wt/vol) NaCl. Pictures were taken 24 h after infiltration and are representative of at least three independent experiments.