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Effects of addition of intermediates with different concentrations on propionic acid production. Intermediate addition experiments were carried out in 100-ml anaerobic jars containing 50 ml culture medium with 30 g/liter CaCO3. Inocula (10%) were transferred to the culture jars for 160 h at 32°C. Black columns, dry cell weight (DCW); red columns, lactic acid (LA); blue columns, acetic acid (AA); purple columns, propionic acid (PA).
