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. 2015 Mar 12;81(7):2256–2264. doi: 10.1128/AEM.03572-14

TABLE 1.

Strains and plasmids used in this studya

Strain or plasmid Relevant characteristic(s) Source or reference
Strains
    E. coli JM110 dam and dcm mutant; host for vector preparation Stratagene
    K. pneumoniae subsp. pneumoniae ATCC 12657 Genomic DNA for gldA, mdh, and fumC ATCC
    P. jensenii ATCC 4868 Host for gene expression and PA production ATCC
Plasmids
    pZGX04 Shuttle vector between P. jensenii and E. coli; Ampr Cmr 13
    pZGX04-gldA gldA-overexpressing plasmid 13
    pZGX04-mdh mdh-overexpressing plasmid This work
    pZGX04-fumC fumC-overexpressing plasmid This work
    pZGX04-gldA-mdh gldA- and mdh-overexpressing plasmid This work
    pZGX04-gldA-fumC gldA- and fumC-overexpressing plasmid This work
    pZGX04-mdh-fumC mdh- and fumC-overexpressing plasmid This work
a

Ampr, ampicillin selection; Cmr, chloramphenicol selection; ATCC, American Type Culture Collection, Manassas, VA, USA.