TABLE 1.
Strain or plasmid | Relevant characteristic(s) | Source or reference |
---|---|---|
Strains | ||
E. coli JM110 | dam and dcm mutant; host for vector preparation | Stratagene |
K. pneumoniae subsp. pneumoniae ATCC 12657 | Genomic DNA for gldA, mdh, and fumC | ATCC |
P. jensenii ATCC 4868 | Host for gene expression and PA production | ATCC |
Plasmids | ||
pZGX04 | Shuttle vector between P. jensenii and E. coli; Ampr Cmr | 13 |
pZGX04-gldA | gldA-overexpressing plasmid | 13 |
pZGX04-mdh | mdh-overexpressing plasmid | This work |
pZGX04-fumC | fumC-overexpressing plasmid | This work |
pZGX04-gldA-mdh | gldA- and mdh-overexpressing plasmid | This work |
pZGX04-gldA-fumC | gldA- and fumC-overexpressing plasmid | This work |
pZGX04-mdh-fumC | mdh- and fumC-overexpressing plasmid | This work |
Ampr, ampicillin selection; Cmr, chloramphenicol selection; ATCC, American Type Culture Collection, Manassas, VA, USA.