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Oligonucleotides and conditions used for PCR in this study
| Primer target (gene) or name | Nucleotide sequence (5′→3′) | Annealing temp (°C) | Extension time (min) |
|---|---|---|---|
| mdh | ATGAAAGTTGCAGTCCTTGGCG | 58 | 1 |
| TTACTTGTTGACGAAGTCTTCGCC | |||
| fumC | ATGACAACGCATCGCAGTGAAAAA | 60 | 1.5 |
| CTAGCGAATAGCCAGGCTGCCG | |||
| pZGX04-orf2 | CGGGGTACCTGGACGATTGAGTACACCGAC | 56 | 8 |
| CCATCGATCATCACGCACCTCCTGACTAC | |||
| pZGX04-orf4 | GACCCACCCTCCCATTCTGTACCC | 60 | 8.5 |
| CATCCTCAGTGCTCGGTGGGG |
