FIG 3.

Results of the cbbL cloning approach, based on deduced cbbL protein sequences. (A) Dendrogram based on Bray-Curtis similarity values; (B) phylogenetic affiliation and relative frequency of the different sequence types. Closest relatives were determined based on BLAST results and distances calculated in ARB. The upper aquifer included site H4-3, and the lower aquifer included sites H4-1 and H5-1. Similarities of deduced cbbL protein sequences obtained in this study to cultivated strains are as follows: 95.6 to 100% for Sulfuricella denitrificans, 95.5 to 98.3% for Sideroxydans lithotrophicus ES-1, 96.7 to 99.4% for Nitrosomonas sp. Is79A3, 94.4 to 96.1% for Acidithiobacillus ferrivorans, 92.2 to 96.7% for Thiobacillus denitrificans, 92.2 to 95.5% for Thiobacter subterraneus, and 91.6% for Thioalkalivibrio sulfidiphilus.