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Evolved strains adapted to higher growth temperatures. Shown are growth curves of GLY3 (closed squares), trm2 (closed circles), tam44 (open triangles), and tam45 (open diamonds) with standard deviations (n = 3). (A) Strains grown in nutrient-rich medium at 33°C (solid lines) or 41°C (dotted lines). (B) Strains grown in minimal medium at 33°C (solid lines) or 38°C (dotted lines).
