. 2015 Mar 12;81(7):2284–2298. doi: 10.1128/AEM.03973-14

TABLE 2.

Primers used to validate mutations in evolved strains

Name	Sequence (5′→3′)	Target	Size (bp)
dR1F	ATGGAACGTTTCCGAAGAGG	Δregion 1	5,296^a
dR1R	TCAGACAGAGTCTTGGTAGC	Δregion 1	5,296^a
dR2F	GGGCTCACTCGAGGGATCTTGACTG	Δregion 2	8,066^a
dR2R	GGCTGTGTACACGTCAGTGAGTCTC	Δregion 2	8,066^a
0035_F	TCGGCTTGGATGGAAAACCT	CgR_0035::1043C→T	385
0035_R	TCCAACCAAATAGACCAATGCTCG	CgR_0035::1043C→T	385
0141_F	TCGGGTGCGTAATCGGAAAC	CgR_0141::470A→G	235
0141_R	GGGATGGTCGCCTAGAGTG	CgR_0141::470A→G	235
0489_F	TCTGGCTGAGCTTCCAGAAG	hemC::574G→A	308
0489_R	GTTGAGCGCGCGGACGGT	hemC::574G→A	308
1044_F	ACCGATGCCCGTGAACTC	glmU::883C→T	394
1044_R	TTTCTACGAAGCCACCGAGC	glmU::883C→T	394
1064_F	TGGTTCTCATGCCCGGAAAC	CgR_1064::767G→A	351
1064_R	GGCCCGAGAAGTCAAGGAAA	CgR_1064::767G→A	351
1240_F	GTAGGGCTCTCATCGCAGG	mhpA::1074G→A	262
1240_R	ATGCGCTCATCGCGCAAA	mhpA::1074G→A	262
1294_F	AACTTCGACCTCGGTGAGGA	nucS::482G→A	388
1294_R	TGGACTCAATGCCACGAAGC	nucS::482G→A	388
2048_F	TTGCGCGTTGAGGTGAACAA	mraW::848G→A	321
2048_R	GTCTCCTGAGTTGTTGGCGA	mraW::848G→A	321
2057_F	GTGCCGGTTAATTCCGCAG	pknL::1099A→G	496
2057_R	GTTCAGCCACTGCCACGAAA	pknL::1099A→G	496
2303_F	ACACGGAGGATGCTGTTTGG	CgR_2303::497C→T	802
2303_R	ACGGGGCGTTTTCTTCTAGC	CgR_2303::497C→T	802
2333_F	TACAACGGTTACCCTGCGGA	CgR_2333::566A→G	365
2333_R	GTGTCCGTACCTAGCACAGG	CgR_2333::566A→G	365
2531_F	GGGCGTTCCCGATCTCGATT	otsA::983G→A	472
2531_R	AAAGCACCAGTGCCGTCG	otsA::983G→A	472
2654_F	CGTGGTGCTCAAAGGCATGA	pknG::431A→G	107
2654_R	CCTTCACGATACCGGGGTG	pknG::431A→G	107
2975_F	CGTCCTTCAGGTCAGAACCA	CgR_2975::185A→G CgR_2976::161T→C	156
2975_R	TTTCTCAGAAGCTTGGCCCT	CgR_2975::185A→G CgR_2976::161T→C	156
1704_2/44_F	TGAAATCCAAAAGCTGCAGGAC	secN::308T→C	274
1704_2/44_R	ATTCTCAGGATGTCCGTCAGTG	secN::308T→C	274
1704_45_F	TCTAGCCATTTTTATCGTTCCCACC	secN::68C→T	110
1704_45_R	TGACAATGCGGTCGCCTG	secN::68C→T	110
2163_2/44_F	ATCGCCGACATTTTGCTTAGTG	hrcA::85A→G	291
2163_2/44_R	ATCAACAAAAAGGCGGTAACCC	hrcA::85A→G	291
2163_45_F	CACTGGGCGGATTGGGG	hrcA::994C→T	110
2163_45_R	CTATTCGCCAGCGAGCACA	hrcA::994C→T	110
2547_2/44_F	GACATTTTCGTCCCGCTGATTC	ptsS::481G→A	276
2547_2/44_R	GTTAACCAGGGTTGGGAACAC	ptsS::481G→A	276
2547_45_F	AGATCAGCGGTGTTGCTGAG	ptsS::1019G→A	797
2547_45_R	GAGTGCAATCAAAGCGCCAC	ptsS::1019G→A	797
1015_1016_F	ATCACGACCACCACTGTACC	Intergenic mutation between CgR_1015 and CgR_1016	404
1015_1016_R	CTCGGCATATCCACTGCCAC	Intergenic mutation between CgR_1015 and CgR_1016	404
1377_1378_F	CTACATGAGCCCGTACATTT	Intergenic mutation between CgR_1377 and CgR_1378	358
1377_1378_R	GGTACTCCGCATCATGTATT	Intergenic mutation between CgR_1377 and CgR_1378	358
1414_1415_F	AGTACATACAGCCGGAGCGA	Intergenic mutation between CgR_1414 and CgR_1415	541
1414_1415_R	TACTGCAGGTCCGATGAGGT	Intergenic mutation between CgR_1414 and CgR_1415	541
1462_1463_F	TTCATCGATAGGGTGGGGCT	Intergenic mutation between CgR_1462 and CgR_1463	402
1462_1463_R	CGAGGGAAACTGCGATGACT	Intergenic mutation between CgR_1462 and CgR_1463	402
2959_2960_F	ACACCACCTCTCTTTTCAACATGA	Intergenic mutation between CgR_2959 and CgR_2960	293
2959_2960_R	TGAGGGCGACTCCAAGTCATA	Intergenic mutation between CgR_2959 and CgR_2960	293

PCR product size is shown for the WT.